Akif Ciftci
University of Freiburg
5 Papers
Akif Ciftci is an academic researcher from University of Freiburg. The author has contributed to research in topics: Transfer RNA & Protein aggregation. The author has an hindex of 4, co-authored 5 publications. Previous affiliations of Akif Ciftci include University of Kassel.
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Papers
tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast
TL;DR: Surprisingly, combined modification defects in the anticodon loops of different tRNAs induce similar cell polarity- and nuclear segregation defects that are accompanied by increased aggregation of cellular proteins.
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Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
Dominik Jacob,Kathrin Thüring,Aurellia Galliot,Virginie Marchand,Adeline Galvanin,Akif Ciftci,Karin Scharmann,Michael Stock,Jean-Yves Roignant,Sebastian A. Leidel,Yuri Motorin,Raffael Schaffrath,Roland Klassen,Mark Helm +13 more
TL;DR: A hybridization‐based approach that uses microscale thermophoresis (MST) as a very fast and highly precise readout to quantify, for example, single tRNA species with a turnaround time of about one hour.
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Loss of Elongator- and KEOPS-Dependent tRNA Modifications Leads to Severe Growth Phenotypes and Protein Aggregation in Yeast
Leticia Pollo-Oliveira,Roland Klassen,Nick Davis,Akif Ciftci,Jo Marie Bacusmo,Maria Martinelli,Michael S. DeMott,Thomas J. Begley,Peter C. Dedon,Raffael Schaffrath,Valérie de Crécy-Lagard +10 more
- 18 Feb 2020
TL;DR: Proteomic analysis of the t6A-deficient strain (sua5 mutant) revealed a global mistranslation leading to protein aggregation without regard to physicochemical properties or t 6A-dependent or biased codon usage in parent genes, but loss of sua5 led to increased expression of soluble proteins for mitochondrial function, protein quality processing/trafficking, oxidative stress response, and energy homeostasis.
22
Absolute Quantifizierung nicht‐kodierender RNA‐Spezies mittels Mikroskala‐Thermophorese
Dominik Jacob,Kathrin Thüring,Aurellia Galliot,Virginie Marchand,Adeline Galvanin,Akif Ciftci,Karin Scharmann,Michael Stock,Jean-Yves Roignant,Sebastian A. Leidel,Yuri Motorin,Raffael Schaffrath,Roland Klassen,Mark Helm +13 more
Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae.
David Maria Hollenstein,Rubén Gómez-Sánchez,Akif Ciftci,Franziska Kriegenburg,Muriel Mari,Raffaela Torggler,Mariya Licheva,Fulvio Reggiori,Claudine Kraft +8 more
TL;DR: Investigation of the role of the PAS–vacuole connection for bulk autophagy in the yeast Saccharomyces cerevisiae reveals that the spatial regulation of autophagosome formation at the vacuole is required for efficient bulk autophileagy.