5 Papers
62 Citations
Akemi Ito is an academic researcher from National Institute of Radiological Sciences. The author has contributed to research in topics: Mutant & Methyl methanesulfonate. The author has an hindex of 4, co-authored 5 publications.
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Papers
Optimum conditions for electric pulse-mediated gene transfer to mammalian cells in suspension.
Hiroko Hama-Inaba,Mitsuko Takahashi,Michiki Kasai,Tadahiro Shiomi,Akemi Ito,Fumio Hanaoka,Koki Sato +6 more
TL;DR: A pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells, and Saline G was better than PBS(-) for obtaining a large number of transformants because it ensured high cell viability.
X-ray-sensitive mutants of mouse mammary carcinoma cells are hypersensitive to bleomycin and hydrogen peroxide.
Koki Sato,Akemi Ito,Tadahiro Shiomi,Hiroko Hama-Inaba,Hiroko Ishikawa,Takeo Yoshizumi,Tohru Nakazawa +6 more
TL;DR: SX9, SX10 and M10 cells were found to be far more sensitive than the respective wild-type cells to hydrogen peroxide inactivation, but the levels of catalase activity were similar among these cell strains.
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Electric Pulse-Mediated Gene Transfer in Mammalian Cells Grown in Suspension Culture
TL;DR: A high-voltage generating machine which could generate semi-rectangular pulses in PBS solution was constructed, and the effects of field strength and duration of the pulse on electric pulse-mediated transformation of mouse mammary carcinoma FM3A cells by a linear form of plasmid pSV2neo DNAs were examined.
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Cross-sensitivity to DNA-damaging agents in radiation-sensitive mutants of murine leukemia cells.
TL;DR: Results indicate that M10 and LX830 have a common defect in the repair of damage caused by X rays, bleomycin, Ara-C and aphidicolin but a different defect inThe repair of MMS-, MMC-, 4NQO-, hydroxyurea and 3-aminobenzamide-damage.
Mutagen detection with a mouse line containing 3 distinct mutations conferring sensitivity
TL;DR: A mouse-cell mutant sensitive to methyl methanesulfonate, X-rays, ultraviolet light (UV), and crosslinking agents was selected using the replica plating and cell suspension spotting methods and showed that low concentrations of mutagens were detected with the multiple mutagen-sensitive mutant XUM1.
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