Ai Ching Lim
Amgen
4 Papers
5 Citations
Ai Ching Lim is an academic researcher from Amgen. The author has contributed to research in topics: Endoplasmic reticulum & Secretory pathway. The author has an hindex of 4, co-authored 4 publications.
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Papers
Topogenesis and cell surface trafficking of GPR34 are facilitated by positive-inside rule that effects through a tri-basic motif in the first intracellular loop.
TL;DR: The placement of a cleavable N-terminal signal sequence as a surrogate topogenic determinant overcame the effects of tri-basic motif mutations and rectified the TM1 orientation; thereby restored the conformational epitope, post-translational modifications, and cell surface trafficking altogether in human GPR34.
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Overexpression of cryoglobulin-like single-chain antibody induces morular cell phenotype via liquid-liquid phase separation in the secretory pathway organelles.
TL;DR: The identification and characterization of a single‐chain antibody (scFv–Fc) that recapitulates cryoglobulin‐like properties is reported, indicating that overproduction of condensation‐prone secretory proteins that culminate in LLPS in the endoplasmic reticulum therefore serves as a path to produce morular Russell body phenotype.
Modulation of in vivo IgG crystallization in the secretory pathway by heavy chain isotype class switching and N-linked glycosylation
Haruki Hasegawa,Carla Forte,Irene Barber,Shanon Turnbaugh,Janelle Stoops,Min Shen,Ai Ching Lim +6 more
TL;DR: An IgG's in vivo crystal morphology and crystallization propensity can be modulated by the properties genetically and biochemically encoded in the HC constant region, and alterations to the constant domain-encoded properties revealed their modulatory roles in CB-inducing propensities and CB morphology.
Russell body phenotype is preferentially induced by IgG mAb clones with high intrinsic condensation propensity: Relations between the biosynthetic events in the ER and solution behaviors in vitro
TL;DR: The findings implicated that RB formation represents a phase separation event or a loss of colloidal stability in the secretory pathway organelles, and the process of RB induction allows the cell to preemptively reduce the extracellular concentration of potentially pathogenic, highly aggregation-prone IgG clones by selectively storing them in the ER.