https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2876%2980445-0

Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex.

Chapter 27 – Electrokinetic Behavior of Red Cells

Human and rabbit red blood cells, separated into "young" and "old" age groups by differential flotation on phthalate esters, were fixed with glutaraldehyde and labeled with colloidal ferric oxide. Electron micrographs of thin sections of young cells showed a uniform and dense depostion of positive iron particles. Old cells showed particles deposited irregularly, leaving unlabeled gaps on the membrane surface. Red cells incubated with 10 units/ml receptor-destroying enzyme (RDE) demonstrate a reduced labeling, similar to that of old cells. After neuraminic acid had been removed from red cells by 20 units/ml RDE, no iron particles were found on membrane surfaces. The different labeling of young, old, and RDE-treated human and rabbit red cells was correlated with their electric mobility and agglutinability by poly-L-lysine. The contradiction between the apparent similarity in charge density of human and rabbit red cells as estimated by density of iron particles and the markedly lower electric mobility of rabbit red cells is discussed.

/pdf/electron-microscope-analysis-of-young-and-old-red-blood-x4wmjt3uyk.pdf

Electron microscope analysis of young and old red blood cells stained with colloidal iron for surface charge evaluation.

Agglutination by polylysine of young and old red blood cells.

The Influenza Viruses

Une methode est decrite qui permet de mesurer et d'enregistrer automatiquement la vitesse d'agglutination des globules rouges par les hemagglutinines virales, la poly-l-lysine et les antiserums. Le fragiligraphe modele D2 muni d'un nouvel accessoire a ete utilise. La difference de vitesse d'agglutination entre cellules jeunes et agees a ete enregistree.

Red cell agglutination kinetics: a method for automatic recording with the fragiligraph.

Une methode est decrite pour la mesure des hemagglutinines virales et des anticorps inhibiteurs des hemagglutinines au moyen d'un systeme photoelectrique et enregistreur automatique — le fragiligraphe. Une suspension des erythrocytes et du virus est introduite dans la microcuvette du fragiligraphe. L'agglutination et la sedimentation des erythrocytes causent l'augmentation de la transmission de la lumiere a travers la suspension. Le temps jusqu'au debut de l'inflexion de la courbe et son angle permettent d'evaluer la concentration du virus.

Rapid titration of viral hemagglutinins and hemagglutination inhibition antibodies with the aid of a fragiligraph.

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Red cell agglutination kinetics: a method for automatic recording with the fragiligraph.

Rapid titration of viral hemagglutinins and hemagglutination inhibition antibodies with the aid of a fragiligraph.