A B Shapiro
Cornell University
4 Papers
159 Citations
A B Shapiro is an academic researcher from Cornell University. The author has contributed to research in topics: Adenine nucleotide & Binding site. The author has an hindex of 4, co-authored 4 publications.
Chat about Author
Papers
Substrate binding-induced alteration of nucleotide binding site properties of chloroplast coupling factor 1.
A B Shapiro,Richard E. McCarty +1 more
TL;DR: Using AMP-PNP, a model in which binding of substrate Mg2(+)-ATP, not hydrolysis, causes the putative catalytic sites to switch properties is supported, in agreement with the alternating site catalytic cooperativity hypothesis.
50
Fluorescence resonance energy transfer mapping of the fourth of six nucleotide-binding sites of chloroplast coupling factor 1.
TL;DR: Two arrangements of the energy transfer map of coupling factor 1 were found which are compatible with the available data and make different predictions about which sites interact in cooperative catalysis.
47
Four tight nucleotide binding sites of chloroplast coupling factor 1.
TL;DR: All four tight nucleotide binding sites of reductively activated chloroplast coupling factor 1 require about one millimolar nucleotide, suggesting that low affinity binding sites are converted to tight binding via a nucleotidebinding-induced conformational change.
40
Alteration of the nucleotide-binding site asymmetry of chloroplast coupling factor 1 by catalysis.
A B Shapiro,Richard E. McCarty +1 more
TL;DR: The results show that the asymmetry of the nucleotide-binding sites of CF1 is not a permanent feature of the molecule, and suggests that the change that results in switching of nucleotide -binding sites 1 and 3 relaxes in the absence of substrate.
30