1. Availability values for amino acids in nine cereal grains determined by faecal analyses with pigs and by the Silcock available lysine test (Roach et al. 1967) and an in vitro digestibility assay were compared with ileal availability values for the same grains determined with pigs by Taverner et al. (1981 b). 2. There was a significant canonical correlation of ileal and faecal availability values. On average, apparent faecal availability of the indispensable amino acids was 4.2% greater than apparent ileal availability, but the difference was up to 12.6% for threonine. Furthermore, the difference appeared to increase as the digestibility of the grain decreased. 3. Silcock available-lysine values for the cereal grains were unrelated to the lysine truly absorbed by the pig. 4. There were close relationships of ileal protein and lysine availability values of nitrogen digestibility determined by an in vitro assay using pronase enzyme.

/pdf/availability-to-pigs-of-amino-acids-in-cereal-grains-3-a-4b4z8ffuqo.pdf

Availability to pigs of amino acids in cereal grains: 3. A comparison of ileal availability values with faecal, chemical and enzymic estimates

A bioavailable nutrient has the potential to be utilized when ingested. Ideally, the nutrient requirements of animals and the nutrient concentrations of feedingstuffs should be expressed in terms o...

Estimation of bioavailable amino acids in feedingstuffs for poultry and pigs: a review with emphasis on balance experiments

A new method for measuring protein with Coo-massie Brilliant Blue G-250 has been adapted for use as a screening method in a potato tuber protein improvement breeding program. The method is simple, fast and inexpensive, and has successfully estimated the total protein content of a broad range of tuber genotypes having dissimilar amino acid profiles and tuber maturities. Correlation between the Coomassie method and a modified micro-Kjeldahl method, the standard method used in the potato breeding program, was 0.93. Free amino acids and other compounds which interfere with other methods for measuring protein do not interfere with the Coomassie Brilliant Blue procedure.

Rapid estimation of potato tuber total protein content with coomassie brilliant blue G-250

1. Protein digestibility and lysine availability were determined in a range of grain samples using an in vivo digestibility assay calibrated with ileal digestibility values. 2. Mean (+/- SE) values predicted for nitrogen digestibility were 0.92 +/- 0.011 in wheat and 0.88 +/- 0.021 in barely, and the predicted lysine availability in wheat was 0.86 +/- 0.021. 3. Chemical and physical characteristics of the grains were determined and those most closely associated with protein digestibility for wheat were the contents of hemicellulose, neutral-detergent fibre, the bulk density, and to a lesser extent, N and acid-detergent fibre content. These relationships were used to determine prediction equations for the availability lysine content of wheat.

/pdf/availability-to-pigs-of-amino-acids-in-cereal-grains-4-2yu28y1yvw.pdf

Availability to pigs of amino acids in cereal grains. 4. Factors influencing the availability of amino acids and energy in grains.

Estimates were made of the dry matter (DM) yield and N content of cereal grains harvested from trial plots. They included 236 combinations of variety and treatment, with 13 varieties of winter wheat grown under six husbandry regimes, 14 varieties of spring barley under three husbandry regimes and ten varieties of winter barley under 11 husbandry regimes. The husbandry included timing and rate of fertilizer application, use of growth regulator and fungicide, and seed rate.For all three cereals, there were highly significant positive associations between grain N content (g/kg DM) and grain DM yield (t/ha) when individual varieties were grown under different conditions. The regression coefficients were: winter wheat 2·28, spring barley 0·74 and winter barley 1·06. When varieties were compared under constant husbandry, the association between N content and yield was negative; the regression coefficients were: winter wheat – 1·53, spring barley –1·14 and winter barley –1·21.The contents of lysine, threonine, isoleucine and valine were examined in 19 samples of winter wheat, 19 of spring barley and 21 of winter barley. Each cereal type included a comparison of varieties under one husbandry regime and a comparison of husbandry regimes in one variety.The amino acid composition of wheat protein changed little with either variety or treatment. In spring and winter barley, lysine concentration in grain protein (g/16 g N) decreased with increases in grain N which arose from additional fertilizer N. There were similar decreases in threonine, isoleucine and valine in winter barley, but varietal differences in grain N were not associated with significant changes in the amino acid composition of grain protein.The nutritive values of spring barley, winter barley and winter wheat were compared in digestion and N balance studies in growing pigs. A subset often samples was examined which included, for each cereal type, high- and low-protein varieties, each (except for spring barley) grown with high or low rates of applied fertilizer N.Apparent digestibility of dry matter measured at the terminal ileum was similar (0·67–0·70) in all three types of cereal but there was a tendency for the DM of high-protein varieties to be digested better than that of low-protein varieties. Over the whole digestive tract, the apparent digestibility of the DM of wheat was higher (0·83) than that of barley (0·75).The apparent digestion of the starch of all samples was virtually complete (0·98) by the end of the ileum; the remainder was digested in the large intestine.The apparent digestibility of N up to the terminal ileum was significantly higher for wheat than for barley, and significantly higher in high- than in low-protein varieties. Over the whole gastro-intestinal tract, the difference between the cereals was even larger; a greater proportion of wheat N than of barley N was digested in the large intestine.The rate of N fertilizer application did not significantly affect the apparent digestibility of any amino acid but there were significant differences amongst the cereal types in the apparent digestibility of seven amino acids. The amino acids in high-protein varieties were, on average, 6 % more digestible than those in low-protein varieties.When allowance was made for the endogenous flow of amino acids, some of the differences between cereals and between high- and low-protein varieties disappeared but some significant differences remained.The biological value (BV) of wheat protein (0·43) was significantly lower than that of barley protein (mean 0·57). When pigs were fed wheat as the sole protein source, the higher N content of wheat compensated for its lower BV. There were no significant differences in the rates of N retention between pigs fed on wheat or barley.

Varietal Differences in the Nutritive-Value of Cereal-Grains for Pigs

A range of samples embracing 14 varieties from different localities within the United Kingdom, many grown with the application of different known levels of nitrogenous fertilizer, were submitted to proximate analysis, some analysed for total amino acid composition and some evaluated nutritionally in near-practical dietary mixtures with various protein concentrates.

The effect of nitrogen fertilization on the amino acid composition and nutritive value of cereals

Evaluation by a chick growth test of mixtures of protein concentrates with cereals did not always confirm quality predictions based upon amino acid composition, but predictions based upon the content of the first limiting amino acid—the Chemical Score—were more generally useful when the concentrates were fed to chicks or rats as a sole protein source. Predictions involving a number of essential amino acids were no more useful than Chemical Score. A knowledge of amino acid deficiencies alone is not sufficient for the prediction of performance under practical conditions.

The relationship between amino acid composition and nutritive value for plant and animal protein concentrates

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The effect of nitrogen fertilization on the amino acid composition and nutritive value of cereals

The relationship between amino acid composition and nutritive value for plant and animal protein concentrates